Fig. 4.

Analyses of contaminants on purified FeIFNβ fraction. a Agarose 1% gel: viral DNA presence analysis. MK: molecular weight marker (100 pb); 1: negative control (H2O); 2: positive control (AcMNPV DNA); 3: original larval extract; 4: original larval extract post-PD10; 5: purified FeIFNβ fraction. b Western blot: S. frugiperda proteins analysis. 1: original larval extract; 2: passthrough; 3: wash1; 4: wash2; MK: protein marker; 5: wash3; 6: purified FeIFNβ fraction. Western blot was developed with a specific antiserum raised against total S. frugiperda extract