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. 2018 Jul 27;3(2):75–86. doi: 10.1002/btm2.10097

Figure 3.

Figure 3

TGF‐β CAR‐T cells do not respond to the latent form of TGF‐β. Soluble TGF‐β in the active or latent (LAP‐bound) form was added at the specified concentrations to a Jurkat T‐cell line that stably expresses an EGFP reporter driven by an NFAT‐responsive promoter. EGFP expression was quantified by flow cytometry after 17 hr. The TGF‐β CAR‐T cells are only activated by the active form of TGF‐β