Figure 5.

TGF‐β CAR expression and stimulation does not result in preferential expansion of FOXP3⁺ Tregs among CD4⁺/CD25^hi/CD127^‐‐sorted cells. (a) TGF‐β CAR‐transduced or untransduced CD4⁺/CD25^hi/CD127^‐‐sorted cells were expanded with Dynabeads only or Dynabeads plus 5 ng/mL TGF‐β for 20 days. Shown are representative scatterplots of FOXP3 versus CD25 expression corresponding to each transduction and expansion condition. (b) FOXP3 mean fluorescent intensity (MFI) and frequency of FOXP3⁺ cells corresponding to each transduction and expansion condition. Averages of triplicates are shown in (b), with error bars representing ± 1 SD. Statistics are calculated by two‐tailed Student's t test with the Sidak correction for multiple comparisons. * p < .05 and ** p < .01