Figure 6.

TGF‐β CAR‐transduced CD4⁺/CD25^hi/CD127^–‐sorted cells are suppressive when stimulated through the TCR. (a) CFSE‐labeled CD4⁺ Teffs that were not previously activated in vitro were cultured in OKT3‐coated wells with CD28 agonist antibody and either 0 or 5 ng/mL TGF‐β, with or without the addition of TGF‐β CAR‐transduced CD4⁺/CD25^hi/CD127^–‐sorted cells (referred to as TGF‐β CAR‐Tregs) at 1:1 Treg:Teff ratio. Representative histogram overlays of CFSE dilution are shown. (b) Co‐cultures were set up as described in (a), except all wells received Tregs that were either untransduced or transduced with the TGF‐β CAR. Representative histogram overlays of CFSE dilution are shown