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. 2018 Jul 27;8:11314. doi: 10.1038/s41598-018-29617-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fabrication of rat mesenchymal stem cell sheets. (a) Histogram of cell surface markers showing the AD-MSC (upper) and BM-MSC (lower). (b) Differentiation capacity of AD-MSC and BM-MSC for differentiating into adipocytes and osteoblasts with alizarin red. (upper panel; undifferentiated condition, lower panel; differentiated condition). Extracellular calcium deposition in the osteoblasts is shown in red, the accumulation of lipid vacuoles in the adipocytes are shown in red. (c) EGFP signal of SD-Tg AD-MSC and BM-MSC 1 day after seeding on a culture dish. AD-MSC and BM-MSC were used at passages 3. (d) Fabricated AD-MSC and BM-MSC sheets. AD-MSC and BM-MSC sheets by hematoxylin-eosin staining. EGFP-expressing monolayered AD-MSC and BM-MSC sheets. AD-MSC and BM-MSC were used at passages 3 or 4. (e) EGFP protein expression levels in Tg-rat isolated AD-MSC and BM-MSC. All the experiments were performed three times. Green: AD-MSC or BM-MSC from EGFP transgenic rats, Red: EGFP antibody labeled by Alexa Flour 594 Blue: nucleus.