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. 2018 Jul 27;8:11314. doi: 10.1038/s41598-018-29617-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of MSC sheet endoscopic transplantation in electrocoagulation model. (a) Endoscopic imaging of the colon induced by electrocoagulation ulcer (left). and MSC sheets transplanted at the site of induction of electrocoagulation ulcer (middle). The yellow arrow points to the transplantation site. One day after MSC sheets endoscopic transplantation (right). (b) Macroscopic representation of the ulcer formation on colon in 1 day after MSC sheets endoscopic transplantation. The yellow arrow points to the transplanted ulcer site. (c) Histological analysis of colon tissue 0 day after MSC sheets. (d) Histological analysis of colon tissue 1 day after MSC sheets (middle; AD-MSC, lower; BM-MSC) transplantation group. Con; only electrocoagulation, Sham; transplanted without MSC sheets, AD-MSC; AD-MSC sheets transplanted, BM-MSC; BM-MSC sheets transplanted. The red fluorescent signal is an EGFP signal in immunofluorescence, Dark brown stain was EGFP signal in DAB stain. GFP/E-Cad/DAPI; immunofluorescence stain, Brown GFP; immunohistochemistry, HE; Hematoxylin and eosin stain. Bars represent 200 μm.