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. 2018 Jul 27;9:2961. doi: 10.1038/s41467-018-05388-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — The autophagy inhibition caused by high cell density is myosin II –dependent. a Representative LC3-II western-blots of HeLa cells overexpressing either the wild-type or the constitutively active MLC2 (T18D, S19D—mut) construct. The graphs show the LC3-II/GAPDH: means ± s.d. (n = 3; *P < 0.05; two-tailed one sample t-test). b Representative LC3-II western-blots of high confluency HeLa cells overexpressing either the wild-type or the constitutively active MLC2 (T18D, S19D - mut) construct. The cells were exposed to BafA1 (400 nM). The graphs show the LC3-II/GAPDH: means ± s.d. (n = 3; **P < 0.01; two-way ANOVA). c Representative HA-htt Q74 aggregates in high confluency HeLa cells overexpressing either the wild-type or the constitutively active MLC2 (T18D, S19D - mut) construct. The graphs show the percentage of cells with HA-htt Q74 aggregates: means ± s.d. (n = 3; ***P < 0.001, NS, not significant; two-way ANOVA). d Representative LC3-II western-blots of high confluency MCF10A cells overexpressing either the wild-type or the constitutively active MLC2 (T18D, S19D - mut) construct. The cells were exposed to either DMSO or BafA1 (400 nM). The graphs show the LC3-II/GAPDH: means ± s.d. (n = 3; ***P < 0.001, **P < 0.01, *P < 0.05; two-way ANOVA). e Representative LC3-II western-blots of HeLa cells overexpressing control (empty flag), YAP(5SA) or YAP(5SA/S94A) exposed to either vehicle (DMSO) or blebbistatin treatment. Bars represent the mean ± s.d. (n = 3; ***P < 0.001, **P < 0.01, *P < 0.05; two-tailed one sample t-test). f Representative LC3-II western-blots of MCF10A cells overexpressing control (empty flag), YAP(5SA) or YAP(5SA/S94A) exposed to either vehicle (DMSO) or blebbistatin treatment. n = number of independent biological replicates unless otherwise stated