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. 2018 Jul 27;8:11324. doi: 10.1038/s41598-018-29745-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Survival of indicated F. tularensis strains during heat and pH stress. Overnight grown bacteria were diluted in fresh growth media and subjected to stress at 50 °C for 30 min (A) or pH 4.5 for 1 h (B). Survival of each strain was monitored as viable bacteria and plotted as percentage of survival, setting wild-type strains survival as 100%. There was no significant killing of any wild-type strain during the treatment. Data are the average survival percentage for at least three independent experiments for each condition. Values are mean ± SD. **P < 0.01; *** indicates P < 0.001 vs. the corresponding wild-type strain.