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. 2018 Jul 27;8:11324. doi: 10.1038/s41598-018-29745-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Intracellular growth and LDH release from F. tularensis-infected BMDM and J774A.1 cells. Growth of the LVS or SCHU S4 ΔclpB mutants and the corresponding wild type strains were analyzed by lysis of infected BMDM (A) and J774A.1 (C) cells at 0 h and 18 h and the number of CFU determined. The net growth mean values ± SEM of at least three independent experiments are shown. Supernatants of cultures of BMDM (B) and J774A.1 (D) cells infected with the indicated strains were harvested at 18 h. The activity was expressed as a percentage of the level of uninfected lysed cells (positive lysis control). The mean values ± SEM of triplicate wells from one representative experiment of two are shown. The asterisk indicates that the values of the ∆clpB mutants differed significantly compared to wild type strains (*P < 0.05; **P < 0.01; ***P < 0.001). Unpaired t-test with Welch’s correction was used to compare values.