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. 2018 Jul 27;8:11324. doi: 10.1038/s41598-018-29745-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Assessment of the phagosomal membrane integrity of BMDM cells infected with indicated F. tularensis strains by the TEM assay. BMDM cells were infected for 2 h at an MOI of 1,000, washed, further incubated for 3 and 6 h, and then processed for TEM. To examine the membrane integrity of phagosome, at least 100 bacteria from different sections were analyzed for each time point and categorized as follows: Intact, >90% of the phagosomal membrane intact; Slightly damaged, 50–90% of the membrane intact; Highly damaged, 10–50% of the membrane intact; No membrane, <10% of the membrane intact.