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. 2018 Jul 27;8:11324. doi: 10.1038/s41598-018-29745-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative examples of secretion of β-lactamase-tagged IglE protein in J774A.1 macrophages. Macrophages were infected either with LVS, LVS ∆clpB, SCHU ∆ggt or SCHU ∆clpB expressing IglE-TEM fusions for 18 h and the fluorescence was determined. TEM β-lactamase activity was identified by the blue fluorescence emitted by the cleaved CCF2 product, whereas uncleaved CCF2 substrate emitted a green fluorescence. The experiments were repeated at least 4 times using duplicate samples. Representative images of each of 4 samples are shown.