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. 2018 Jul 27;8:11324. doi: 10.1038/s41598-018-29745-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Replication of F. tularensis strains in liver and spleen following infection of mice. After intradermal inoculation with 1 × 10⁴ CFU of the indicated strain, mice were sacrificed on day 5, 9, 15 and 21, and bacterial burdens in spleen and liver were determined. The mean ± SEM for six mice per group and time point are shown. A significant difference in the bacterial numbers of mutant strains vs. LVS is indicated as follows: *P < 0.05; **P < 0.01; ***P < 0.001; NS P > 0.05.