Table 3.
Kinetics of carbonylated proteins production on C. elegans after acute irradiation at 2.5 Gy (1 Gy.min−1) and at 75 Gy (15 Gy.min−1).
| Time post irradiation (h) | Control | 1 h | 1h30 | 2 h | 2h40 | 3 h | 4 h | 5 h | 6 h |
|---|---|---|---|---|---|---|---|---|---|
| 2.5 Gy | 1 ± 0.1 | 1.2 ± 0.2 | 0.9 ± 0.1 | 1.6 ± 0.4 | NA | 2.0 ± 0.7* | NA | 1.9 ± 0.7 | 1.3 ± 0.2 |
| 75 Gy | 1 ± 0.2 | 1.2 ± 0.2 | 0.8 ± 0.2 | NA | 2.0 ± 0.2*** | NA | 1.9 ± 0.1*** | 2.1 ± 0.3*** | NA |
Worms used in this assay were 3 hours synchronized, irradiated at 2.5 and 75 Gy, and collected at different times post irradiation as indicated. n = 3 (pool of 1000 nematodes). Data are expressed as induction (ratio of carbonylated protein level between exposed and controls) of carbonylated proteins compared to controls as means ± SD (*P < 0.05; **P < 0.01; ***P < 0.001, Dunnet test). NA corresponds to No Available Data.