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. 2018 Jul 27;8:11340. doi: 10.1038/s41598-018-29583-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Mounted sample in the self-locking clamps (top right), and schematic representation of the preparation table (center of image) including a (enlarged) molding tool for the determination of cross sections of the samples prior to testing (right bottom). (B) Clamp designs with 4-sided pyramid structures in cross-sectional view. Pyramid dimensions (d = diagonal, h = height) are enlarged. Additionally, corresponding scanning electron microscopy images of clamped ligament tissues after testing at 25x magnification are represented at the right. Note the elongated impressions in the ligament tissues clamped with the sharp pyramids (scale bar 100 μm).