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. 2018 Jul 27;8:11340. doi: 10.1038/s41598-018-29583-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Steps of trimming, mounting and casting specimens. (1) The cutting template consists of a bottom and a top plate (not shown), and a guide rail allowing for exact positioning. Radii and lengths can be adjusted according to the ISO standard to get a ‘dog bone’ shape. Removed tissues can be used for further analyses, e.g. on ultrastructure or water content. (2) Each clamp consists of four identical parts interlocking pairwise, mounted to the end of the specimen pairwise in a fixed sample length given by the mounting Table. (3) Casts of the specimens’ cross-sectional areas can optionally be made using a designated opening at the mounting table. Impression material may either be directly placed in this opening, or in a two-part impression device shown in the picture, allowing for less use of the impression material.