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. 2018 Jul 27;8:11340. doi: 10.1038/s41598-018-29583-4

Table 4.

Comparison of four clamping techniques and their suitability for biomechanical testing.

Sand paper clamping Partial plastination Cryogenic clamping 3D printed clamping system
Level of standardization (e.g. specimen alignment, clamping length) Low Moderate Moderate High
Ease of use Easy Complex Moderate Easy
Use of potentially dangerous goods? No Yes (solvents & resin) Yes (liquid N2) No
Adaptability to various tissues None None Moderate High
Modularity None None None High
Material slippage High Minute Low Low
Work time involved for preparation excluding testing 15 mins/sample 30 mins/sample 15 mins/sample 10–15 mins/sample
Applicability for quasi-static testing Suitable Suitable Suitable Suitable
Applicability for cyclic and fatigue testing Unsuitable (material slippage) Unsuitable (avulsion at the clamping interface) Unsuitable (continuous cooling necessary, temperature decrease of tissues) Suitable for ≥105 cycles
Throughput high low moderate high
Type & price of consumables and mounting materials per specimen Sandpaper
ca. 0.30 NZ$
(0.18 US$/€)
Acetone, resin
5–7 NZ$
(3–4 US$/€)
Liquid nitrogen
1 NZ$/l
(0.60 US$/l)
Printing materials
ca. 0.50 NZ$
(0.30 US$/€)
Reusability of consumables not given not given not given estimated 3–5×*
Desinfectability poor poor good good
Sterilizability poor poor good poor
Suitable field of application Preliminary tests Highly-specialized applications Small sample sizes, high tensile forces High-standardization-high-throughput experiments

*Applied to aseptic conditions using 50% ethanol or Viraclean®.