Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 27;8:11348. doi: 10.1038/s41598-018-29421-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Mitochondrial morphology, distribution and projection pattern of cortical primary neurons in Wdfy3-haploinsufficient mice. Mitochondrial staining of primary cortical neurons from WT (A,C) or Wdfy3-haploinsufficient mice (B,D) was performed with MitoTracker and images recorded with an Olympus FV1000 confocal microscope at 60X magnification, as described in the Methods Section. A tubular, elongated pattern is evident in mitochondria from WT (C, insets) whereas Wdfy3^+/lacZ neurons show fewer, smaller mitochondria and budding protrusions (D, insets). Overlapping projections (panels A,B, circled in red) were visualized with ImageJ and their frequencies counted manually. (E,F) Quantification of the mitochondria content [percentage of cellular area covered by mitochondria, (E)] and mitochondrial morphology (F) were performed used a macro for ImageJ (see Methods). (G) Number of networks per cell and number of branches per network were evaluated with the MiNA macro (see Methods). Statistical analysis was performed with a 2-tailed Mann-Whitney test. Significance was considered with p values < 0.05.