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. 2018 Jul 27;8:11339. doi: 10.1038/s41598-018-29477-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The phenotypic analysis of cultured ASCs. The results are based on 10 biological replicates, including 5 plastic surgery and 5 oncological surgery patients. Flow cytometry assessment of both positive (A) and negative (B) ASCs markers showed statistically significant changes in the expression of the following positive markers: ANPEP (CD13), CD44, NT5E (CD73) and ENG (CD105); and two negative surface markers of ASC – PECAM1 (CD31) and CD34 (all marked with*). The X axis represents the passage number (from P1 to P6), whereas the Y axis displays the median fluorescence intensity (MFI) of cells expressing particular surface marker. The Wilcoxon signed-rank test was used to compare the dynamics of changes between passages. Statistical significance was accepted when P was ≤ 0.01. Center lines denote the median, box limits indicate the 25th and 75th percentiles; whiskers represent the maximum and minimum of the acquired values.