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. 2018 Jul 27;9:2960. doi: 10.1038/s41467-018-05376-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — 3D tissue packing of curved epithelia. a Example of Drosophila salivary gland and its processed images. Scale bar = 100 μm. b Confocal images showing the apico-basal cell intercalation of epithelial cells marked with green, yellow, red, and blue pseudo-colours. The green cell participates in two apico-basal transitions. c 3D reconstruction of the same cells labelled in b using the same colour code. The image confirms the presence of concave surfaces predicted by the mathematical model