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. 2018 Jul 27;8:11341. doi: 10.1038/s41598-018-29744-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Metabolite features whose level varies significantly (p < 0.01) across groups are projected on the cloud plot depending on their retention time (x-axis) and m/z (y-axis). Statistical significance (p-value) is represented by the bubble’s color intensity. The size of the bubble denotes feature intensity (only features with maximum intensity above 1 000 000 are displayed). Feature assignments (p-value, m/z, RT) are displayed in a pop-up window for the identified metabolite tryptophan. (B) Extracted ion chromatogram (EIC) and (C) Boxplot of tryptophan. *p < 0.05 vs respective control.