Figure 1.

Early Neurogenesis Is More Abundant in OB than Adjacent NCx in Mouse Embryo

(A) Schematic of progenitor cell lineages in direct and indirect neurogenesis. N, neuron; CP, cortical plate.

(B) Parasagittal sections of developing mouse telencephalon. Dashed lines indicate extent of the OB. MGE, medial ganglionic eminence; Sp, septum.

(C–F) Distribution and abundance of neurons (Tuj1+ and Tbr1+ cells) in OB and NCx at E12.5, and quantifications as indicated (n = 3–9 embryos per group; t test). ML, mantle layer.

(G) Cumulative BrdU labeling at E12.5 to measure cell cycle length (n = 3 embryos per time-point).

(H and I) Analysis of cell cycle exit at E12.5 (BrdU+Ki67−, arrowheads; n = 3 embryos per group; χ²-test).

(J and K) Distribution of IPCs (Tbr2+Tbr1−, red arrowheads) and neurons (Tbr1+, green arrowheads; or Tbr2+Tbr1+, white arrowheads) at E12.5, and quantifications of Tbr2+ cells, IPCs (E12.5) and basal mitoses at the indicated ages (n = 3-11 embryos per group; t tests for density, χ²-tests for co-localization).

Values are mean + SEM; ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001. Scale bars: 300 μm (B), 50 μm (C–J).

See also Figure S1.