Figure 5.

Robo/Dll1/Jag Signaling Drives Direct Genesis of Deep-Layer Corticofugal Neurons

(A–G) ISH stains for Notch pathway genes in control and Robo1/2^−/− embryos at E12.5, and intensity quantification (paired t test).

(H) ISH stains for Jag1 and 2 in NCx upon electroporation of the indicated plasmid combination (EP), and quantification of intensity (ratio to contralateral non-electroporated hemisphere; paired t test).

(I) WT NCx electroporated with the indicated plasmid combinations, and ratio of density of Tuj1+ cells (red) in the VZ between electroporated and non-electroporated hemispheres (t test).

(J) Experimental design to identify the fate of neocortical neurons born by direct neurogenesis.

(K–M) WT NCx electroporated with the indicated plasmid combinations, and laminar distribution of all GFP+ cells (L), top) and with 100% BrdU label (born by direct mode, arrowheads; [L], bottom; one-way ANOVA followed by χ²-test).

(N–P) Identification of neuron types in WT OB electroporated with the indicated plasmid combinations, and quantification (χ²-test). Left plot is cell types in any layer; right plot is cell types sorted by layers.

Values are mean + SEM, n = 3–5 embryos per group; ^∗’p = 0.050; ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001. Scale bars: 50 μm (A–I, M, and O), 400 μm (K).

See also Figures S6 and S7.