Figure S5.

Regulation of OB Neurogenesis and Growth by Robo Receptors and Slit Ligands, Related to Figure 3

(A) Sagittal sections of the rostral telencephalon showing the growth and evagination of the OB in WT and mutant embryos from E12.5 to E18.5; dashed lines indicate OB perimeter.

(B) External lateral view of the brain in WT (+/+) and Robo1/2^−/− embryos.

(C) Measurement of the OB perimeter on sagittal sections from WT and Robo1/2^−/− embryos from E11.5 to E16.5 (n = 3-9 embryos per group).

(D) ISH on sagittal sections from WT and mutant embryos at E18.5 for the indicated genes, which delineate the extension of the OB by being either selectively expressed (Reln, Grm1, Tbr2) or selectively absent (Bhlhe22) in the mitral cell layer (MCL).

(E–H) Distribution and abundance of Tbr1+ and Tuj1+ neurons (E and G) and PH3+ apical and basal mitoses (F,H) in OB and NCx of WT and Slit1/2^−/− embryos at E12.5. Plots show density of cells per 100 μm of ventricular surface in the VZ (left) and mantle layer (ML) or cortical plate (CP) (n = 3 embryos per group).

Values are mean ± SEM; t tests, ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ns = not significant. Scale bars: 200 μm (A: E12.5, E14.5), 500 μm (A: E16.5, E18.5; D), 1 mm (B), 50 μm (E and F).