Table 1.
Univariate analysis factors associated with PFS or OS
| OS | PFS | |
|---|---|---|
| HR (95% CI) P-value |
HR (95% CI) P-value |
|
| Clinical parameters | ||
| Age, (≤ 65 v > 65) | 1.50 (0.84–2.67) P = 0.170 |
1.39 (0.81–2.39) P = 0.230 |
| Sex, (Male v Female) | 1.18 (0.68–2.06) P = 0.559 |
1.061 (0.66–1.81) P = 0.829 |
| TNM stage, (I + II vs. III + IV) | 2.76 (1.45–5.24) P = 0.002 |
2.10 (1.18–3.73) P = 0.012 |
| Risk factors | ||
| Gall stone (no v yes) | 1.58 (0.89–2.81) P = 0.117 |
1.47 (0.85–2.54) P = 0.166 |
| Diabetes (no v yes) | 1.27 (0.66–2.45) P = 0.467 |
1.15 (0.64–2.05) P = 0.640 |
| Hypertension (no v yes) | 1.40 (0.77–2.52) P = 0.270 |
1.50 (0.86–2.63) P = 0.156 |
| Cholecystitis (no v yes) | 1.34 (0.74–2.41) P = 0.335 |
1.34 (0.77–2.35) P = 0.304 |
| Jaundice (no v yes) | 2.82 (1.49–5.33) P = 0.001 |
2.58 (1.35–4.92) P = 0.004 |
| Completed resection (no v yes) | 0.38 (0.21–0.68) P = 0.001 |
0.40 (0.22–0.72) P = 0.002 |
| CEA (> 5 μg/L) | 1.97 (1.08–3.60) P = 0.028 |
1.61 (0.90–2.89) P = 0.110 |
| CA19–9 (> 34 U/mL) | 2.77 (1.52–5.06) P = 0.001 |
2.28 (1.28–4.04) P = 0.005 |
| Liver dysfunction | ||
| ALT (> 40 U/L) | 2.13 (1.15–3.95) P = 0.016 |
2.04 (1.10–3.79) P = 0.023 |
| AST (> 35 U/L) | 1.63 (0.91–2.91) P = 0.099 |
1.34 (0.79–2.42) P = 0.264 |
| TBil (> 22.2 μmol/L) | 1.625 (0.91–2,90) P = 0.101 |
1.38 (0.79–2.44) P = 0.261 |
| DBil (> 6.8 μmol/L) | 2.71 (1.50–4.91) P = 0.001 |
2.45 (1.35–4.47) P = 0.003 |
| GGT (> 45 U/L) | 2.34 (1.31–4.17) P = 0.004 |
2.055 (1.17–3.61) P = 0.013 |
| ALP (> 135 U/L) | 1.99 (1.12–3.56) P = 0.020 |
1.75 (0.99–3.08) P = 0.055 |
| Pathological features | ||
| Differentiation (moderate vs. poor) | 1.95 (1.10–3.46) P = 0.023 |
1.57 (0.92–2.68) P = 0.100 |
| Mean tumor size (≤ 3.0 v > 3.0 cm) | 1.07 (0.61–1.88) P = 0.816 |
1.02 (0.60–1.72) P = 0.955 |
| Immunohistochemistry | ||
| CD133+ in TCs | 1.09 (0.62–1.91) P = 0.776 |
0.99 (0.58–1.71) P = 0.994 |
| CD8+ TILs (CD8low vs. CD8high) | 0.49 (0.28–0.86) P = 0.014 |
0.54 (0.31–0.93) P = 0.026 |
| PD-1+ in TILs | 1.14 (0.66–1.98) P = 0.636 |
1.26 (0.74–2.13) P = 0.398 |
| PD-L1+ in TCs | 1.92 (0.95–3.88) P = 0.067 |
1.87 (0.95–3.68) P = 0.071 |
| PD-L1− vs. PD-L1+ | 1.86 (1.04–3.33) P = 0.038 |
1.60 (0.93–2.75) P = 0.088 |
| PD-L2- vs. PD-L2+ | 1.52 (0.82–2.80) P = 0.185 |
1.40 (0.80–2.46) P = 0.242 |
| Classification based on PD-L1 expression and CD8+ TILs | ||
| Overall | NA; P = 0.003 | NA; P = 0.018 |
| I vs. II | 3.14 (1.11–8.85) P = 0.031 |
2.40 (1.03–5.59) P = 0.042 |
| I vs. III | 3.36 (1.21–9.30) P = 0.020 |
2.75 (1.16–6.51) P = 0.021 |
| I vs. IV | 7.38 (2.49–21.89) < 0.001 |
4.81 (1.81–12.78) P = 0.002 |
NOTE: Univariate analysis, Cox proportional hazards regression model