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. 2018 Jul 28;18:80. doi: 10.1186/s12866-018-1216-6

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Localization of loaded DNA in L. Casei ghosts visualized by confocal laser scanning microscopy. a L. casei 393 ghosts filled with SYBR Green I labeled pCI-EGFP under white light. b L. casei ghosts filled with SYBR Green I labeled pCI-EGFP. Overlay of differential interference contrast and fluorescent image. The image indicated that the plasmid was filled within the bacterial ghosts. c Ghost membranes were stained with MitoTracker Green FM (green). d pCI-EGFP was detected by in situ hybridization with Cy3-labeled probes (red) specific for the EGFP. e All fluorescence photomicrographs (c, d) were taken of one middle z-scan section through the middle plane of the loaded bacterial ghosts and subsequently overlaid (e). Direct overlays of red and green fluorescent structures are represented by the yellow color