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. 2018 Jul 28;18:80. doi: 10.1186/s12866-018-1216-6

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Determination of DNA loaded in the BGs by qPCR. L. casei 393 ghosts were loaded with pCI-EGFP plasmid. BGs were washed five times with HBS after loading with pCI-EGFP. DNA in bacterial ghosts and washing solution were extracted respectively and quantified by qPCR. Each point was the mean of quadruplicate measurements ± SD. Each experiment was repeated at least twice