FIGURE 1.

Ganciclovir (GCV) treatment terminates murine cytomegalovirus (MCMV) replication and is associated with reduction of CD45+ leukocyte infiltrates into the allograft. (A) DNA was extracted from whole blood of CMV-infected animals with and without GCV treatment at 14 days posttransplant, quantitative DNA PCR performed using primers, probe and standards specific for MCMV immediate-early 1, and results depicted as copies per mL blood. MCMV DNA was consistently detectable in blood from CMV-infected transplant recipients without GCV (CMV, closed squares) but was not detectable (nd) in animals treated with GCV (GCV). (B) CD45+ leukocytes were quantitated by flow cytometry from allografts of uninfected (Mock, open squares), CMV-infected (CMV, closed squares), and CMV-infected, GCV-treated (GCV, hatched squares) animals, and depicted as cells per organ. CMV-infected allografts showed greater CD45+ infiltrates compared with Mock allografts (P<0.01). CD45+ leukocytes in the GCV allografts were decreased compared with CMV allografts (P<0.05) but were still greater (P<0.01) than numbers found in Mock allografts.