Skip to main content
. Author manuscript; available in PMC: 2019 Oct 5.
Published in final edited form as: Gene. 2018 Jun 18;673:95–101. doi: 10.1016/j.gene.2018.06.048

Fig. 1.

Fig. 1

The human genome encodes a FOXO3A-related transcript, FOXO3B. A. BLAT alignment of the ZNF286A, FOXO3A and FOXO3B transcripts to the FOXO3B (aka ZNF286B) locus on 17p11.2 (Hg38 assembly) with the mammalian conservation track shown (UCSC Genome Browser). Red hash marks within the aligned sequence indicate sequence mismatches between the aligned transcript sequence and the genome. B. Protein sequence alignment of FOXO3A and predicted FOXO3B protein. The blue bar above the sequence indicates the forkhead domain, the red asterisk the FOXO3B stop codon and the red ‘P’ the FOXO3A Akt phosphorylation site (Thr32 FOXO3A, Thr117 FOXO3B). C. RT-qPCR measurement of FOXO3B expression across a panel of human cell lines and tissues normalized to 18S rRNA and calculated relative to the expression in fetal liver using the ΔΔCt method. Errors bars are SD of three replicates.