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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: J Immunol. 2018 Jun 22;201(3):971–981. doi: 10.4049/jimmunol.1701551

Figure 3. NK cells from ART-suppressed individuals respond to combined blockade of the PD-1 and IL-10 pathways.

Figure 3.

(A-E) PBMCs from ART donors were stimulated in vitro with an HIV Gag peptide pool or left unstimulated, this in the presence of blocking antibodies against PD-L1 and IL-10Rα or corresponding isotype controls. A delayed ICS assays performed after 48h. (A-D) Summary data of the frequencies of (A) IFN-γ-producing NK cells, (B) IFN-γ/CD107a co-expressing NK cells and (C) TNF-α-producing NK cells and (D) IFN-γ-CD4 T cells. (E) Fold increase in net cytokine expression upon PD-L1/IL-10 dual blockade in ART donors compared to untreated individuals presented in Figure 2. Number of ART subjects: (A-E) n=6. Wilcoxon paired test (a A-D). Non-parametric Mann-Whitney U test (p˂0.05) (E).