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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: J Immunol. 2018 Jun 22;201(3):971–981. doi: 10.4049/jimmunol.1701551

Figure 5. Combined blockade of the PD-1 and IL-10 pathways restores NK cell killing.

Figure 5.

CD8-depleted PBMCS were incubated overnight with an HIV Gag peptide pool, SEB or left unstimulated in the presence of isotype control antibodies or blocking antibodies against PD-L1 or IL-10Rα. On the next day, the PBMCs were mixed with target K562 cells, with effector and target cells identified by cell tracker dyes. Killing was measured by a live/dead dye. (AB) Representative examples of (A) the gating strategy used and (B) results obtained in the killing assay. (C) Summary of the pooled data of the specific killing of K562 cells observed in the Unstimulated, Gag-stimulated and SEB-stimulated conditions. (D) Increase in specific killing elicited by combined PD-1/IL-10 blockade in the Gag-stimulated conditions. Statistical tests: (C) Non-parametric Friedman test for multiple paired comparisons with Dunn’s post tests; (D) Wilcoxon matched-pairs test. * p<0.05; ** p<0.01; *** p<0.001; **** p<0.0001.