Table 2. Changes of differentially expressed proteins of E. coli TG1 following exposure to cadmium.
Protein | Up(+)/Down(−) regulation (fold) | ||||
---|---|---|---|---|---|
TG1 carrying pUC19 plasmid | TG1 expressing GFP | TG1 expressing His6GFP | TG1expressing OmpA | TG1 expressing His-OmpA | |
Energy metabolism | |||||
Glycolysis | |||||
Glyceraldehyde-3-phosphate dehydrogenase (1) | −2.02 | −1.84 | Absence | +2.69 | Absence |
Glyceraldehyde-3-phosphate dehydrogenase (2) | +1.75 | +1.65 | −1.06 | −2.29 | +1.63 |
Tricarboxylic acid cycle | |||||
Aconitase | −1.20 | No change | Absence | No change | Absence |
Glycerol uptake and lipolysis | |||||
Glycerol kinase | −1.60 | +1.73 | +1.92 | +1.43 | +1.33 |
Protein biosynthesis machinery | |||||
Translation elongation factor-Ts | +2.12 | +1.84 | +1.35 | −0.39 | +1.37 |
Transporters | |||||
Oligopeptide-binding protein | −1.65 | +1.37 | −1.34 | +1.22 | +1.21 |
Amino acid ABC transporter substrate-binding protein | −1.42 | +1.37 | +1.29 | Not detectable | +1.24 |
H+-transporting ATPase | +2.69 | −1.09 | +1.30 | −2.64 | No change |
ATP synthase F1, beta subunit | +1.32 | +1.26 | +1.08 | +1.86 | −1.44 |
Stress defense mechanism | |||||
MnSOD | +2.20 | +1.29 | −1.65 | Not detectable | +2.08 |
Alkyl hydroperoxide reductase | −1.15 | +1.71 | −1.10 | +1.52 | −1.01 |
DNA starvation/stationary phase protection protein; Dps | −3.29 | Low amount | −1.35 | Low amount | −1.05 |
Others | |||||
Ribosomal protein L9 | +2.08 | +1.33 | −1.29 | +1.42 | +1.57 |
GFP | Not detectable | −1.45 | Not detectable | Not detectable | Not detectable |
His6GFP | Not detectable | Not detectable | −2.24 | Not detectable | Not detectable |
Disulfide isomerase I | −1.71 | Not determined | −1.85 | Not determined | −1.36 |
OmpA (1) | −1.68 | −1.26 | −1.40 | −1.35 | Absence |
OmpC precursor | −1.15 | −1.31 | −1.56 | +1.32 | +2.11 |
OmpF | −1.18 | Absence | Absence | No change | Absence |