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. Author manuscript; available in PMC: 2019 Jul 17.
Published in final edited form as: Immunity. 2018 Jul 17;49(1):42–55.e6. doi: 10.1016/j.immuni.2018.06.011

Figure 5. Casp8 regulates inflammatory signaling in ileum.

Figure 5

(A) Immunoblot of ileal mucosa from unmanipulated (time=0) or from WT and Casp8−/−Ripk3−/− mice at the indicated mpc with LPS, showing expression of phosphorylated forms (p) of IκBα (40 kDa), SAPK and JNK (54 and 46 kDa, respectively), as well as Cl-C8, C8, cFLIPL (50 kDa) and cFLIPs (25 kDa), RIPK1 (75 kDa), IgH (50 kDa), C3, C11, C1 (43 kDa) and Gsdmd (55 kDa, with an * indicating the ~30 kDa cleaved form). (B) Principal component (PC) analysis (PCA) of ileal mucosa from WT, Casp8−/−Ripk3−/− and Casp11−/− mice (n=5 for each group) including 27,578 genes with detectable base mean expression where the contribution of each PC is indicated as a percentage. (C) Heat map comparing expression patterns of genes in ileal mucosa from WT, Casp8−/−Ripk3−/− and Casp11−/− mice (n=5 for each group) at 1.5 hpc, where mean log2 expression of each gene was compared to normalized expression of all genes. Blue, white and red indicate downregulated, unchanged and upregulated expression of genes, respectively. Please also see Figure S5.