Examples of chemical signals that can be generated from our acoustofluidic chemical signal generator. Chemical signals with a wide temporal range can be generated solely by modulating the driving signals of the transducer. (A) Single-pulse signals with stimulation durations of 100 ms, and 1, 5 and 10 sec. (B) Periodic chemical signals with a constant period of 50 sec and various durations of 100 ms, and 1, 5 and 10 sec. (C) Periodic chemical signals with a fixed duty cycle of 50 % and varying periods of 2, 4, 8 and 10 sec.