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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Dev Biol. 2018 Jul 11;441(1):132–145. doi: 10.1016/j.ydbio.2018.06.020

Figure 1. Smo acts with Ush to block lamellocyte differentiation and is required to maintain Ush expression levels.

Figure 1

(A–D) Smo acts with Ush to block lamellocyte (lm) differentiation. Lymph glands from (C) ush/smo double heterozgyotes had significantly increased numbers of lymph gland lobes with lamellocyte differentiation compared to either (A) smo heterozygotes or (B) ush heterozygotes. Lamellocytes were detected using the MSN-C reporter and are marked with arrows. (D) Histogram showing the results of the statistical analyses. Chi-square test; P value is as shown; smo (n=214); ush and ush/smo (n= 26). (E–G) Loss of Ush function has no effect on Smo expression. Smo expression in (F) ushvx22/r24 {ush hypomorphs) was assessed and compared to (E) wild-type controls (+). (G) Histogram showing the results of the statistical analyses. Student’s t-test; error bars show standard deviation; P values are as shown; control and ush hypomorphs (n = 15). (H–J) Loss of Smo function (SmoDN) significantly reduced Ush levels compared to controls (+). Tep-Gal4 females were crossed to control (+) males or males that carry UAS-smoDN. (J) Histogram showing the results of the statistical analyses. Student’s t-test; error bars show standard deviation; P values are as shown; control and SmoDN (n=18). White dotted lines delineate the entire lymph gland. Scale bars: panels A–C, 100 pm; remaining panels, 50 μm. Panels A–D, late-third instar larvae; panels E–J, mid-third instar larvae.