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. 2018 Jul 23;9:1721. doi: 10.3389/fimmu.2018.01721

Figure 5.

Figure 5

In vitro effects of plasma from healthy controls or patients with C1-INH-HAE on vascular permeability. (A) Bovine aortic endothelial cells (BAEC) were incubated (18 h, 37°C) with plasma from healthy controls or from symptom-free patients with C1-INH-HAE. The in vitro vascular permeability was assessed as indicated in Section “Materials and Methods.” (B) Plasma of patients with C1-INH-HAE was incubated (20 min, 37°C) with anti-VEGF-A (1 µg/ml), anti-Ang2 (1 µg/ml), anti-Ang1 (1 µg/ml), Me-Indoxam (100 nM), RO032107A (100 nM), or control medium. BAEC were then pre-incubated (30 min, 37°C) with heparinase (0.4 U/ml) or control medium and stimulated (18 h, 37°C) with plasma of C1-INH-HAE patients alone or with the combination of C1-INH-HAE plasma with inhibitors and then we evaluated vascular permeability. Data are shown in relative fluorescence units. *p Value ≤0.05 and **p value ≤0.01 vs untreated plasma.