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. 2018 Jul 23;9:1720. doi: 10.3389/fimmu.2018.01720

Figure 1.

Figure 1

Lack of glucocorticoid-induced leucine zipper (GILZ) in B cells increases B cell abundance, but not for the B220+CD5+CD1dhi [regulatory B (Breg)] subset. (A) Blood cell counts in aged (7- to 8-month olds) wild-type (WT) and gilz B cKO mice; abbreviations in x-axis indicate different blood cell population, as follows: WBC, white blood cells; NE, neutrophils; Ly, lymphocytes; MO, monocytes; EO, eosinophils; BA, basophils. (B) Total number of cells in the spleen of WT and gilz B cKO mice. (C) Flow cytometry analysis of B220+ expression in the spleen of WT and gilz B cKO mice. The graph on the right represents the frequency of B220+ cells in the spleen. (D) Number of leukocyte subsets, reported as B220+ B cells (B220), CD4+ T cells (CD4), CD8+ T cells (CD8), Mac1+ Gr1− monocytes/macrophages (Mac1), Cd4+FoxP3+ regulatory T cells (Tregs), in the spleen of WT and gilz B cKO mice (n = 14, 15). (E) Flow cytometry analysis of Breg cells in the spleen of WT and gilz B cKO mice. (F) Frequency and number of Breg cells in the spleen of WT and gilz B cKO mice (n = 12). N.S., not significant; statistical analysis was performed using the unpaired Student’s t-test (*p < 0.05, **p < 0.005, ***p < 0.001).