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. 2018 Jul 23;9:1720. doi: 10.3389/fimmu.2018.01720

Figure 2.

Figure 2

Lack of glucocorticoid-induced leucine zipper (GILZ) in B cells leads to increased production of IFN-γ in splenic B cells. (A) Flow cytometry analysis of IFN-γ, interleukin (IL)-17, IL-10, and IL-4 ex vivo production by B220+ cells in the spleen of wild-type (WT) and gilz B cKO mice. Plots in the left panels are representative of flow cytometry analysis of IFN-γ production by B220+ cells. (B) Quantitative real-time PCR analysis of cytokine produced by B220+ cells purified from spleen tissue stimulated in vitro with phorbol 12-myristate 13-acetate/ionomycin and lipopolysaccharide for 24 h. Data are derived from three independent experiments. (C) Concentration of IFN-γ, IL-17, IL-10, and IL-4 measured by enzyme-linked immunosorbent assay in sera of 9- to 12-month-old WT and gilz B cKO mice (n = 7, 7). N.S., not significant; statistical analysis was performed using the unpaired Student’s t-test (*p < 0.05, ***p < 0.001).