Skip to main content
. 2018 Jul 23;9:1720. doi: 10.3389/fimmu.2018.01720

Figure 3.

Figure 3

Lack of glucocorticoid-induced leucine zipper (GILZ) in B cells leads to increased production of IFN-γ by splenic T cells and in peripheral blood. (A) Analysis of IFN-γ cytokine production by CD4+ cells in the spleen of wild-type (WT) and gilz B cKO mice. (B) Cell counts of WT, gilz B cKO B, and T cells co-cultured and stimulated in vitro with phorbol 12-myristate 13-acetate (PMA)/ionomycin and lipopolysaccharide (LPS) for 24 h. (C,D) Flow cytometry analysis of IFN-γ production in B cells [(C), B220+] or T cells [(D), CD4+] co-cultured and stimulated in vitro with PMA/ionomycin and LPS for 24 h. (E) Flow cytometry analysis of IFN-γ production in B and T cells co-cultured and stimulated in vitro with PMA/ionomycin and LPS for 24 h with or without anti-IFN-γ antibodies (Ra4 and XGM; n = 9, 10); N.S., not significant; statistical analysis was performed using the unpaired Student’s t-test (*p < 0.05, **p < 0.005).