Skip to main content
. 2018 Jul 23;9:1720. doi: 10.3389/fimmu.2018.01720

Figure 4.

Figure 4

Lack of glucocorticoid-induced leucine zipper (GILZ) in B cells increases the production of IFN-γ via enhanced AP-1 transcriptional activity. (A,B) Chromatin immunoprecipitation analysis of the ifng promoter in spleen cells of wild-type (WT) and gilz B cKO mice with anti-c-Jun antibody (A) or anti-acetylated histone H3 [(B); n = 9]. Statistical analysis was performed using the unpaired Student’s t-test (**p < 0.005). (C) Western blot analysis of nuclear c-Jun levels in purified B cells from spleens of WT and gilz B cKO mice. The graph on the right shows the results of densitometry analysis of c-Jun relative to the housekeeping gene lamin B1. Statistical analysis was performed using the nonparametric Mann–Whitney U test (*p < 0.05).