Table 1.

Fluorescently tagged misfolding model proteins.

Name	Origin	Mutant model	Misfolding	Control	Expression	Fluorescent tag location	Reference
Luciferase	P. pyralis	Heat denaturation	Ts	N/A	Constitutive – ACT1	N-terminus	Specht et al., 2011
FlucSM	P. pyralis	Heat denaturation/missense	Ts	Fluc	Constitutive	C-terminus	Ruan et al., 2017
ubc9-2	S. cerevisiae	Heat denaturation/missense	Ts	UBC9	Induced – GAL	N-terminus	Kaganovich et al., 2008
guk1-7	S. cerevisiae	Heat denaturation/missense	Ts	GUK1	Constitutive – TDH3	C-terminus	Comyn et al., 2016
gus1-3	S. cerevisiae	Heat denaturation/missense	Ts	GUS1	Constitutive – TDH3	C-terminus	Comyn et al., 2016
pro3-1	S. cerevisiae	Heat denaturation/missense	Ts	PRO3	Constitutive – TDH3	C-terminus	Comyn et al., 2016
ugp1-3	S. cerevisiae	Heat denaturation/missense	Ts	UGP1	Constitutive – TDH3	C-terminus	Comyn et al., 2016
Actin(E364K)	D. melanogaster	Missense	Continuous	None available	Induced – GAL	C-terminus	Kaganovich et al., 2008
VHL	H. sapiens	Absent binding partner	Continuous	N/A	Induced – GAL	N-terminus	Kaganovich et al., 2008
ΔssCPY∗	S. cerevisiae	Missorting	Continuous	N/A	Constitutive – PRC1, Induced – GAL	C-terminus	Park et al., 2007, 2013
ΔssPrA	S. cerevisiae	Missorting	Continuous	N/A	Constitutive – TDH3	C-terminus	Prasad et al., 2010
tGnd1	S. cerevisiae	Nonsense	Continuous	GND1	Constitutive	C-terminus	Miller et al., 2015
DegAB	S. cerevisiae)	Degron (contains degradation signal)	Continuous	N/A	Constitutive – TDH3	N-terminus	Shiber et al., 2013
Htt103Q	H. sapiens	Huntington’s disease	Continuous	Htt25Q	Induced – GAL	C-terminus	Krobitsch and Lindquist, 2000
β-amyloid	H. sapiens	Alzheimer’s disease	Continuous	N/A	Induced – GAL	C-terminus	Treusch et al., 2011
Alpha synuclein	H. sapiens	Parkinson’s disease	Continuous	N/A	Induced – GAL	C-terminus	Outeiro and Lindquist, 2003
FUS	H. sapiens	Amyotrophic lateral sclerosis (ALS)	Continuous	N/A	Induced – GAL	C-terminus	Fushimi et al., 2011; Kryndushkin et al., 2011; Sun et al., 2011
TDP-43	H. sapiens	Amyotrophic lateral sclerosis (ALS)	Continuous	N/A	Induced – GAL	C-terminus	Johnson et al., 2008; Kryndushkin et al., 2011
OPTN	H. sapiens	Amyotrophic lateral sclerosis (ALS)	Continuous	N/A	Induced – GAL	C-terminus	Kryndushkin et al., 2012

References are to the earliest use of the misfolded reporter in the study of spatial quality control.