Fig. 1.
Functional characterization of HEK-ACΔ6 and HEK-ACΔ3/6 cell lines. Parental HEK293 cells and the CRISPR/Cas9 based HEK-ACΔ6 and HEK-ACΔ3/6 cells were incubated at room temperature with increasing concentrations of the direct AC activator, forskolin (A), or the Gαs-coupled receptors agonists isoproterenol (B) or PGE2 (C), and cAMP accumulation was measured. *HEK-ACΔ6 and HEK-ACΔ3/6 responses to PGE2 overlap in (C). Data represent the mean and S.E.M. of three to four independent experiments conducted in duplicate.