Fig. 3.
Gαs-coupled receptor-mediated activation of the nine mACs in the HEK-ACΔ3/6 cell line. HEK-ACΔ3/6 cells were transiently transfected with Venus or the individual mAC isoforms as indicated. After 40 hours, cAMP accumulation was stimulated with 10 μM isoproterenol (A) or 10 μM PGE2 (B). Data represent the mean and S.E.M. of three to four independent experiments conducted in duplicate. Statistical analysis was performed using paired t test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 compared with the cAMP levels of the vehicle-treated cells.