Fig. 7.

Cyclic AMP responses to forskolin and A23187 of AC1 mutants expressed in HEK293 and HEK-ACΔ3/6 cells. HEK293 (A) or HEK-ACΔ3/6 cells (B) were transiently transfected with Venus, AC1-wt, or an AC1 mutant construct, as indicated. After 40 hours, cAMP accumulation was stimulated with increasing concentrations of forskolin in the absence (red) or presence (blue) of 3 μM A23187. Black and green circles represent the forskolin response of Venus-transfected cells in the presence or absence of A23187, respectively. Data represent the mean and S.E.M. of three independent experiments conducted in duplicate.