Fig. 6.

(A) Quaternary ammonium compound 8. (B) Effect of a single aerosolized dose of 8 (18 nmol/kg) on the recruitment of eosinophils to the airways following aerosol challenge with mixed HDM allergens in sensitized brown Norway rats. The compound was administered 2 hours prior to HDM allergen by means of a Penn-Century aerosolizer (Penn-Century, Philadelphia, USA). Data are displayed as means ± S.E. in groups of 10 animals. *P < 0.05 vs. vehicle (veh) challenge; **P < 0.05 vs. HDM without test substance pretreatment; veh, vehicle. Full details of treatment protocols are provided by Newton et al. (2014) and the online supporting information, https://pubs.acs.org/doi/suppl/10.1021/jm501102h and https://pubs.acs.org/doi/suppl/10.1021/jm501102h/suppl_file/jm501102h_si_001.pdf. BAL, bronchoalveolar lavage.