Skip to main content
. 2018 Apr 11;63(1):58–65. doi: 10.3164/jcbn.17-111

Fig. 6.

Fig. 6

Effects of conditioned medium from PFD-treated NR8383 cells on Col1a1 and HSP47 mRNA expression in RLFs. RLFs were incubated at 37°C for 24 h. RLFs were stimulated with either 10% cell-free medium or conditioned media from NR8383 cells pre-treated with the indicated concentrations of PFD. Col1a1 mRNA (a) and HSP47 mRNA (b) expression were measured by real-time PCR and values were normalized to GAPDH. Treatments are as described Fig. 5. Each bars represents the following; open bar: cell-free medium; shaded bar: conditioned medium from naïve cells; closed bar: conditioned medium from NR8383 cells stimulated with IL-4 + IL-13; hatched bars: conditioned medium from NR8383 cells stimulated with IL-4 + IL-13 and indicated concentrations of PFD. Data are means ± SD from six experiments. #p<0.05 and ##p<0.01, when compared with A group and *p<0.01, when compared with C0 group.