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. 2018 Jul 12;7(14):e005171. doi: 10.1161/JAHA.116.005171

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© 2018 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Schematic illustration of the protocol used for ischemia/reperfusion‐injured (I/R‐injured) rat heart. A, Schematic illustration of the protocol for I/R‐injured rat heart in vitro using the Langendorff perfusion system. After 30 minutes of stabilization, the isolated Langendorff‐perfused hearts were subjected to 40 minutes of global ischemia followed by 60 minutes of reperfusion. Gastrin: infusion of gastrin before ischemia for 20 minutes. CI988+gastrin: infusion of CI988 and gastrin before ischemia for 20 minutes. LY294002+gastrin: infusion of LY294002 and gastrin before ischemia for 20 minutes. PD98059+gastrin: infusion of PD98059 and gastrin before ischemia for 20 minutes. S3I‐201+gastrin: infusion of S3I‐201 and gastrin before ischemia for 20 minutes. B, Schematic illustration of the protocol used for I/R‐injured rat heart in in vivo experiment. I/R‐injury was induced by occlusion of the left anterior descending coronary artery for 30 minutes with a small microvascular clamp and then released for 24 hours. 0.9%NS+I/R indicates intraperitoneal injection of normal saline before ischemia/reperfusion; CI988+gastrin+I/R, intraperitoneal injection of CI988 and gastrin before ischemia/reperfusion; gastrin+I/R, intraperitoneal injection of gastrin before ischemia/reperfusion.