Figure 4.
Protective effects of gastrin on H/R‐injury: Primary cultured cardiomyocytes were pretreated with gastrin (0, 10−10, 10−9, 10−8, 10−7, and 10−6 mol/L) before H/R treatment (A). *P<0.007 vs 0 mol/L group. Data are presented as mean±SEM, n=6. Gastrin attenuated I/R‐induced cardiomyocyte apoptosis: Representative images (B1) and quantification (B2) of in situ TUNEL (terminal deoxynucleotidyl transferase dUTP nick‐end labeling) assay of primary cultured cardiomyocytes. TUNEL‐positive is green and DAPI is blue. Scale bar=400 μm. Cardiomyocyte apoptosis was also analyzed by Western blotting of cleaved and total caspase 3 (C). *P<0.01 vs control; # P<0.01 vs H/R, 1‐way ANOVA, Holm–Sidak test. Data are presented as mean±SEM, n=6 per group. DAPI indicates 4′,6‐diamidino‐2‐phenylindole; H/R, hypoxia/reoxygenation; 0 mol/L group, group without gastrin.