Skip to main content
. 2018 Jul 9;7(14):e009289. doi: 10.1161/JAHA.118.009289

Figure 9.

Figure 9

Suppression of mitochondrial respiration by adenovirus overexpression of D7‐Des in NRCs. A, Mitochondrial OCR profile in NRCs infected with D7‐Des adenovirus at 2.5, 5.0, and 10.0 multiplicities of infection or control adenovirus (β‐Gal). Arrows indicate the sequential addition of oligomycin (1 μmol/L), FCCP (4 μmol/L), and rotenone (0.5 μmol/L) plus antimycin A (0.5 μmol/L). OCR profile is expressed as pmol O2/min per μg of protein. Graph showing OCR at (B) baseline as well as with the addition of (C) oligomycin, (D) FCCP, and (E) rotenone plus antimycin A. Key parameters of mitochondrial function include (F) reserve capacity, (G) ATP turnover, and (H) maximal respiration. Bars represent mean±SEM. n=5 wells per group. P values were determined by Tukey's post hoc test. D7‐Des Tg indicates mutant desmin transgenic mouse; FCCP, carbonyl cyanide‐p‐trifluoromethoxy‐phenylhydrazone; NRCs, neonatal rat cardiomyocytes; NS, not significant; OCR, oxygen consumption rate.