Figure 10.
Effects of mitochondrial fission inhibitor mdivi‐1 on mitochondrial respiration in NRCs. A, Mitochondrial oxygen consumption rate (OCR) profile in NRCs treated with 1, 5, and 10 μmol/L mdivi‐1 treatment for 24 hours in NRCs. Arrows indicate the sequential addition of oligomycin (1 μmol/L), FCCP (4 μmol/L), and rotenone (0.5 μmol/L) plus antimycin A (0.5 μmol/L). OCR profile is expressed as pmol O2/min per μg of protein. Graph shows OCR at (B) baseline as well as with the addition of (C) oligomycin, (D) FCCP, and (E) rotenone plus antimycin A. Key parameters of mitochondrial function including (F) reserve capacity, (G) ATP turnover, and (H) maximal respiration were not significantly changed by mdivi‐1 treatment. I, LDH release in the medium with increasing dose of mdivi‐1. Bars represent mean±SEM. n=5 wells per group. P values were determined by Tukey's post hoc test. FCCP indicates carbonyl cyanide‐p‐trifluoromethoxy‐phenylhydrazone; LDH, lactate dehydrogenase; NRC, neonatal rat cardiomyocyte.