Figure 11.

Mitochondrial fission inhibition by mdivi‐1 in D7‐Des overexpressed NRCs. A, Representative Western blot and densitometric quantification showing Drp1, OPA1, and PGC1α expression after 5 μmol/L mdivi‐1 or vehicle for 24 hours in D7‐Des overexpressed cardiomyocytes. B, Expression of Bax and CHOP in the whole cell lysate, and (C) LDH release in the medium after 5 μmol/L mdivi‐1 or vehicle treatment. D, Desmin expression in the pellet fraction was significantly decreased after mdivi‐1 treatment. E, Desmin expression in the whole cell fraction was not significantly changed after mdivi‐1 treatment. Ponceau S protein stain of the transfer membrane was used to confirm approximately equal loading. n=3 replicates per group. Boxes represents interquartile ranges, lines represent medians, whiskers represent ranges, and P values were determined by Kruskal–Wallis test. LDH indicates lactate dehydrogenase; MOI, multiplicities of infection; NRCs, neonatal rat cardiomyocytes; NS, not significant.